Fig. 1.

Conotoxin cDNAs identified in the cDNA library of C. flavidus. A, distribution percentage of different conotoxin superfamilies in the ConoServer database, the conotoxins identified in the C. flavidus cDNA library, and the conotoxins identified via MS/MS in the venom of C. flavidus. B, comparison of the cysteine codon usage of V-conotoxins from C. flavidus with cysteine framework XV or VI/VII. The TGC cysteine codons are shown in light gray, and the TGT codons are shown in dark gray. C, comparison of the precursor sequences of two A-superfamily conotoxins from C. flavidus demonstrates that the alteration in the cysteine framework in Fla6.1 is caused by a fragment insertion.