Table I. Conotoxins identified via confident MS/MS analysis.

Superfamily	cDNA	Precursor sequence	Conotoxin sequence	Conotoxin name	Monoisotopic mass (MH+)	Mass deviation (ppm)	MS/MS spectra
A	Fla1.3/1.4/1.5	MGMRMMFTMLLLVVLATTVVSFTLDHAFDGRN(I/T)AANNKATDLMALPVRGCCSDPPCRHKHQDLCG	↓CCSDPPCRHKHQDLC*	fla1a	1968.7996	2.44	Supplemental Fig. S3
A			GCCSDPPCRHKHQDLC*	fla1b	2025.8185	3.65	Supplemental Fig. S4
B	Fla-5	MQLYTHLYLLVPLVAFHLILGTGTLAHGDALTERRSADATALKPEPVLLQKSAARSTDDNDRDRLTQRKRILKKQGNMARGYEEDREIAETIRELNEAGK	GYEEDREIAETIREL↓ (four of these five Glu residues are carboxylated, the positions of which are not identified)	Conantokin-fla	1998.8379	1.58	Supplemental Fig. 2A
L	Fla14.3	MKLSVTFIVVLMLTTSLTFGFSLSSNNGERAYGSHHSYVADQLVRRERASRACNPPCSDILTCLHGTCKHLGIG	ACNPPCSDILTCLHGTCKHLGI*	fla14a	2523.1657	2.64	Supplemental Fig. S5
L			ACNPOCSDILTCLHGTCKHLGI*	fla14b	2539.1588	3.31	Supplemental Fig. S6
L			ACNOOCSDILTCLHGTCKHLGI*	fla14c	2555.1532	3.50	Supplemental Fig. S7
L	Fla14.2	MKLSVTFIVVLMLTTSLTFGFSLSSNNGERAYGPRHSNVADQLVPREQASRTCYPPCIGYTYCKSGTCEYRQGRASTVFLKRQ	TCYPPCIGYTYCKSGTCEYRQ*	fla14d	2663.1040	3.94	Supplemental Fig. S8
M	Fla3.4	MMSKLGVLLTISLLLFPLTAVQLDGEQPVDLLALRTQDFAPEQSPWFDPVKRCCSKYCWECTPCCPYSSG	CCSKYCWECTOCCPYSS*	fla3a	2319.7952	4.41	Supplemental Fig. S9
M	Lv3-V04	MLKMGVVLFTFLVLFPLATLQLDADQPRARYAENKQDFNRNERTKMILSAVSASMGRQRRCCLWPACWGCVCCY	↓RCCLWPACWGCVCCY	fla3b	2017.7830	2.66	Supplemental Fig. S10
M	Fla3.2/3.3	MLKMRVVLFTFLVLFPLATLQLDADQPRARYAENKQDFNRNERTKMILSAVSASMGRQRRCCLWPECGGCVCCY	↓RCCLWPECGGCVCCY	fla3c	2036.7294	3.47	Supplemental Fig. S11
M			↓RCCLWPγCGGCVCCY	fla3d	2080.7210	2.44	Supplemental Fig. S12
O1	Fla-17	MKLTCILIVAVLFSTAWTFVTVDDSINHLKYREWTDFRPWGKRIPEARDKMKNLKFSKLDNKKRRL	EWTDFRPW*	fla01	1135.5289	2.71	Supplemental Fig. S13
O1			γWTDFRPW*	fla02	1179.5192	2.16	Supplemental Fig. S14
O1	Fla-18	MKLTCVMIVAALFLTAWTFVTADNSKYELENRGGLGHAGGWVKAGALGKDPGWGKRFLMARDEMKNPKASKLVP	GGLGHAGGWVKAGALG↓	fla03	1407.7446	3.22	Supplemental Fig. S15
O1			GGLGHAGGWVKAGALGKDPGW*	fla04	1990.0303	5.13	Supplemental Fig. S16
O1			GGLGHAGGWVKAGALGKDPGW	fla05	1991.0250	0.26	Supplemental Fig. S17
O1			↓LGHAGGWVKAGALGKDPGW*	fla06	1875.9901	3.97	Supplemental Fig. S18
O1			↓HAGGWVKAGALGKDPGW*	fla07	1705.8867	3.15	Supplemental Fig. S19
O1	/	/	SCGHSGAGCYSRPCCPGLHCSGTHAGGVCV	fla6e	3205.2635	1.89	Fig. 2B
O1	Fla6.4	MKLMCVLIIAVLFLTASQLITADYSRDKQEYRAERLRDAMGKFKGSRSCGHSGAGCYTRPCCPGLHCSGGQAGGLCV	SCGHSGAGCYTRPCCPGLHCSGGQAGGLCV	fla6a	3180.2563	5.66	Supplemental Fig. S20
O1			SCGHSGAGCYTROCCPGLHCSGGQAGGLCV	fla6b	3196.2557	4.23	Supplemental Fig. S21
O1			SCGHSGAGCYTROCCOGLHCSGGQAGGLCV	fla6c	3212.2467	5.44	Supplemental Fig. S22
O1	Fla6.5	MKLTCALIVAMLFLTACQLTTTDDSRGRQKYPTERLRVKMRNPKLSKLTKTCDPPGDSCSRWYNHCCSKLCTSRNSGPTCSRP	TCDPOGDSCSRWYNHCCSKLCTSRNSGOTCSRP	fla6d	3992.5991	1.33	Supplemental Fig. S23
T	Fla5.2	MRCVPVFIILLLLSPSAPSVDAHPKTKDDVPLASFHDDAKRTLQRLWIKALCCYGYAFCCRLGR	ALCCYGYAFCCRL*	fla5a	1712.7104	3.52	Supplemental Fig. S24
T			↓LCCYGYAFCCRL*	fla5b	1641.6746	2.85	Supplemental Fig. S25
T	LiC121 (P01681)	MRCVPVFIILLLLSPSAPSVDAHPKTKDDVPLASFHDDAKRTLQRLWIKALCCYGYRFCCPNFR	ALCCYGYRFCCPN↓	fla5c	1740.6666	4.82	Supplemental Fig. S26
V	Fla15.7	MSTPKMTPFILLLLFSLTIRCGDGKAIQEDRDPSTRLLTGDKNRDLSVNRRCSSCNGEICCGSCFCFPHGKNCFCRQLGK	↓SCFCFPHGKNCFCRQL*	fla15a	2116.9010	3.52	Fig. 3
Q	Fla16.1/16.2	MHTLEMLLLLLLLLPLAPGEGDGQAVAGDRNPSEARSTHEHFLQRLIRLIHGSDCQPCGQYVCCPPWKYAEYRRFT	LIHGSDCQPCGQYVCCPPWKY↓	fla16a	2625.1047	3.59	Fig. 4A
Q			LIHGSDCQPCGQYVCCPPWKYA↓	fla16b	2696.1408	3.86	Fig. 4B
Q			LIHGSDCQPCGQYVCCPPWKYAE↓	fla16c	2825.1839	3.53	Fig. 4C
Q			LIHGSDCQPCGQYVCCPPWKYAEY	fla16d	2988.2481	3.04	Fig. 4D

Notes: O represents hydroxylated proline, and γ is the carboxylated glutamate residue. C-terminal amidation is denoted by an asterisk. Arrows indicate the unexpected proteolytic cleavage.