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. 2013 Oct 29;13(1):184–203. doi: 10.1074/mcp.M113.030155

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Fig. 4. — Immunoprecipitation of host interactor proteins validates their interactions with viral HCV proteins. A, immunoprecipitation of FLAG-tagged host interactor proteins HSD17B12, MTHFD1, XPO1, HBxIP, and FKBP5 coupled to FLAG peptide elution and Western blotting detection of Myc-tagged HCV proteins (reciprocal co-IP). Immunoprecipitation of FLAG-eYFP was used as a negative control. B, immunoprecipitation of endogenous host interactor proteins DDX3X, ILF2, EXOC7, XPO1, MTHFD1, KPNB1, EGLN1, and FKBP5 followed by Western blotting detection of 3xFLAG-tagged HCV proteins (reciprocal co-IP). Immunoprecipitation of Human influenza hemagglutinin (HA) was used as a negative control. Ab, antibody.