Fig. 9.

NS3/4A and its interactor KPNB1 prevent STAT1 nuclear localization. A, Huh7.5 cells were transfected with JFH-1 mutant Δcore 153–167 or NS3+ Q221L. Six days post-transfection, cells were serum starved for 4 h and then stimulated with 5 ng/ml of IFN-γ for 30 min. Cells were fixed, permeabilized, and stained with anti-NS3 or anti-core (red) and anti-STAT1 (green) and then examined via laser-scanning microscopy. B, Huh7 cells were infected with lentiviruses encoding shRNA NT or KPNB1 for three days (MOI = 10). Three days post-infection, cells were serum starved for 4 h and then stimulated with 5 ng/ml of IFN-γ for 30 min. Cells were fixed, permeabilized, and stained with anti-STAT1 (green). Nuclei were stained with Hoechst. Cells were examined via laser-scanning confocal microscopy.