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2DE patterns of total seed proteome (top row) compared with de novo synthesized proteome (bottom row) during Arabidopsis seed germination. Total proteins were separated via 2DE, and the patterns of the neosynthesized proteome were revealed by autoradiography of [35S]-Met pulse-chase time windows (0–8 HAI, 8–16 HAI, 16–24 HAI, 24–32 HAI, 32–40 HAI, and 40–48 HAI) during the time course of seed germination.
