Fig. 3.

K-means clustering of the neosynthesized proteins. Protein radiolabeled intensities corresponding to the same protein were summed when multiple protein isoforms were detected. This allowed us to obtain 158 nonredundant protein radiolabeled intensities. Then, for each protein, the protein neosynthesis intensity was normalized by its maximum value over the whole experiment to get a value between 0 and 1. Finally, a K-means clustering (eight clusters, 10 iterations) based on Pearson correlation was performed on the 158 normalized protein intensities using MEV software (37). The numbers in parentheses indicate the number of nonredundant neosynthesized proteins classified in each cluster.