Table I. Incorporation of [35S]-Met in neosynthesized proteins during the time course of Arabidopsis seed germination.
Hours after imbibition (HAI) | 0–8 | 8–16 | 16–24 | 24–32 | 32–40 | 40–48 |
cpm/μg protein | 48 ± 7 | 531 ± 43 | 498 ± 23 | 322 ± 19 | 353 ± 25 | 293 ± 24 |
Note: After each 8-h time window of [35S]-Met incorporation during seed germination, total proteins were extracted and the amount of [35S]-Met taken into proteins was quantified as counts per minute (cpm) per microgram (μg) of protein.