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Cumulative mortality assay. A, Juvenile P. vannamei were injected intramuscularly with NaCl or dsRNAs (specific to IMNV, WSSV051 and WSSV517 genes) 2 days before WSSV challenge. There were 15 shrimp in each group and experiments were conducted for 17 days. The results are indicated as means with error bars of duplicate experiments. B, Mean time to death was calculated in each treatment group and is shown as mean with standard error (S.E.).
