Table II. Significantly differentially expressed proteins of LIS fraction from vastus lateralis muscle of mature and old women, quantified by label-free mass spectrometry.

Gene	UniProt accession	Protein name	Ratio (Old/Mature women)	Quantitation analysis (p value)	Western-Blot (p value)	Peptides used for quantitation	Mascot Score	Main biological function
ACADM	P11310	Medium-chain specific acyl-CoA dehydrogenase, mitochondrial	−1.16	0.01	0.05	2	119	Energy metabolism
ATP5A1	P25705	ATP synthase subunit alpha, mitochondrial	−1.12	0.01	0.05	3	193	Energy metabolism
ATP5B	P06576	ATP synthase subunit beta, mitochondrial	−1.11	0.04		3	107	Energy metabolism
CRAT	P43155	Carnitine O-acetyltransferase	−1.21	0.001		1	50	Energy metabolism
FH	P07954	Fumarate hydratase, mitochondrial	−1.27	0.004		2	163	Energy metabolism
GOT2	P00505	Aspartate aminotransferase, mitochondrial	−1.22	0.02	0.05	9	575	Energy metabolism
LDHB	P07195	l-lactate dehydrogenase B chain	−1.20	0.046		11	831	Energy metabolism
SLC25A4	P12235	ADP/ATP translocase 1	−1.19	0.03		1	38	Energy metabolism
TALDO1	P37837	Transaldolase	−1.16	0.03		1	75	Energy metabolism
FABP4	P15090	Fatty acid-binding protein, adipocyte	1.69	0.002		2	75	Energy metabolism
PFKL	P17858	6-phosphofructokinase, liver type	1.41	0.03		1	58	Energy metabolism
MYH1	P12882	Myosin-1	−1.16	0.002		2	64	Myofibrillar proteins
MYL1	P05976	Myosin light chain 1/3, skeletal muscle isoform	−1.29	0.003		1	50	Myofibrillar proteins
TTN	Q8WZ42	Titin	−1.19	0.01		8	401	Myofibrillar proteins
ANKRD2	Q9GZV1	Ankyrin repeat domain-containing protein 2	1.25	0.02		2	101	Myofibrillar proteins
ACTB	P60709	Actin, cytoplasmic 1	−1.16	0.01		7	342	Actin microfilament
FLNC	Q14315	Filamin-C	−1.15	0.03		3	148	Actin microfilament
ANXA2	P07355	Annexin A2	−1.18	0.03		2	132	Actin microfilament
CFL1	P23528	Cofilin-1	1.47	0.03		1	43	Actin microfilament
TAGLN	Q01995	Transgelin	1.33	0.03	0.001	1	91	Actin microfilament
BLVRB	P30043	Flavin reductase (NADPH)	−1.30	0.01		1	86	Detoxification, Cytoprotection
HSPA4	P34932	Heat shock 70 kDa protein 4	−1.24	0.01		2	61	Detoxification, Cytoprotection
OAS2	P29728	2′−5′-oligoadenylate synthase 2	−1.14	0.04		1	42	Detoxification, Cytoprotection
CA3	P07451	Carbonic anhydrase 3	1.41	0.04	0.05	16	1464	Detoxification, Cytoprotection
APEH	P13798	Acylamino-acid-releasing enzyme	−1.11	0.03		1	41	Protein turnover
RPS27A	P62979	Ubiquitin-40S ribosomal protein S27a	−1.20	0.0002		2	106	Protein turnover
EEF2	P13639	Elongation factor 2	−1.14	0.048		4	159	Protein turnover
PRKAR2A	P13861	cAMP-dependent protein kinase type II-alpha regulatory subunit	−1.18	0.03		1	62	Signal transduction
YWHAE	P62258	14–3-3 protein epsilon	−1.17	0.0003		7	220	Signal transduction
AHSG	P02765	Alpha-2-HS-glycoprotein	−1.23	0.01	0.05	1	55	Serum
FGA	P02671	Fibrinogen alpha chain	−1.20	0.01		4	142	Serum
HV304	P01765	Ig heavy chain V-III region BRO	−1.19	0.046		1	76	Serum
KV402	P01625	Ig kappa chain V-IV region Len	−1.14	0.02		2	128	Serum
C4A	P0C0L4	Complement C4-A	−1.22	0.002		2	115	Serum
HBG1	P69891	Hemoglobin subunit gamma-1	−1.31	0.002		1	69	Serum