Fig. 3.

Performance metrics for one hour analysis, performed in quintuplicate, of a yeast trypic digest using the Q-OT-qIT hybrid. On average, 3977 yeast proteins were identified in each experiment (1% FDR) with only 13.5% (538, x̄) originating from single peptide identifications (A). 4395 proteins were detected across all experiments—3643 of which were present in all five one hour experiments (B). C, presents the median sequence coverage for the individual and combined experiments. D, displays the overlap in our identified proteins versus known expression level information derived from published tagging experiments.