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. 2013 Oct 8;13(1):63–72. doi: 10.1074/mcp.M113.032235

Fig. 7.

Fig. 7.

Increased ubiquitin E3 ligase activity after S-nitrosylation of RNF11, RNF41 and RNF141. A, In vitro ubiquitination activity assay with or without GSNO treatment for 1 h in presence of ubiquitin, E1 and E2 (UbcH5c) enzymes analyzed by immunoblot with anti-ubiquitin and anti-GST antibodies. B, In vivo ubiquitination activity assay with or without GSNO treatment for 6 h analyzed by immunoblot with anti-HA and anti-V5 antibodies. Actin and HA-ubiquitin expression were monitored as loading controls. All experiments were repeated three times.

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