Fig. 6.

HCV RNA can co-immunoprecipitate with hnRNP K protein. Analysis of hnRNP K binding to HCV replicon RNA was assessed using the CLIP assay (31). A, Western blot analysis of the levels of hnRNP K in Huh7.5 cells with or without HCV replicon. The amount of input lysate loaded in the Western blot analysis was equivalent to 10% of the amounts processed for immunoprecipitation. The sample number at the bottom of the Western blot image is used consistently in all panels of this figure. B, CLIP products from Huh7.5 cells harboring the HCV replicon (lane 4) contained the 5′ UTR of the HCV genome. The image is of a 1.5% agarose gel containing PCR products amplified from the CLIP reaction. The sample identified with a “+” is the amplification of the total lysate from Huh7.5 cells harboring the HCV replicon. C, Quantitative RT-PCR to determine the number of HCV 5′ UTR precipitated from either Huh7.5 cells with or without the HCV replicon. Each sample was from the products of three independent CLIP reactions.