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. 2013 Dec 27;6:58. doi: 10.1186/1755-8794-6-58

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Copyright © 2013 Jones et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Clustering and Principal Component analysis revealed and removed contaminating epigenetic signatures from blood in buccal swabs. a) Dendrogram of relatedness of overall patterns of DNA methylation in all 20 individuals studied. In all figure parts, colours of participant codes indicate Praxis scores, as shown in scale in centre. b) PCA plot showing individual participants’ scores for PC1 (x-axis) and PC2 (y-axis) without correction. c) PCA plot showing scores for PC1 and PC2 for individual participants as well as 10 blood samples. Five DS participants clearly scored intermediate between the other buccal samples and the blood scores for PC1. d) Dendrogram of relatedness of overall patterns of DNA methylation in all 20 individuals studied after removal of PC1 from c. Overall distances between samples were reduced.