Table 2.
Validation of SOLiD based gene expression profiles by Real-Time PCR
| |
|
Log
2
(Average relative expression)
a
|
|
|
|---|---|---|---|---|
| Gene name | Gene function | RT-PCR | SOLiD | Validation (yes/no) |
| Esi0102_0070 |
Arf1, ARF family GTPase |
−1.93 |
−2.05 |
Y |
| Esi0067_0029 |
long chain acyl-coA synthetase |
−4.19 |
0.49 |
N |
| Esi0069_0059 |
Mannuronan C-5-epimerase |
−2.98 |
0.06 |
N |
| Esi0101_0018 |
Tubular mastigoneme-related protein |
6.16 |
4.72 |
Y |
| Esi0104_0023 |
GPCR-like protein |
4.72 |
2.22 |
Y |
| Esi0130_0068 |
PKD/REJ-like protein |
0.62 |
2.22 |
Y |
| Esi0418_0017 |
MORN motif precursor |
4.95 |
32.19 |
Y |
| Esi0123_0020 |
hypothetical protein |
−7.15 |
−31.61 |
Y |
| Esi0161_0002 |
Metal ion transporter-like protein |
3.50 |
−32.10 |
N |
| Esi0098_0063 | hypothetical protein | −4.92 | −31.61 | Y |
aRelative expression is calculated as a ratio of expression levels in male/female gametes to indicate genes up- or downregulated in male gametes. A gene is considered differentially expressed if its relative expression is twofold or greater.