Figure 3.
Silencing of LAMC2 in ATC cells induced cell cycle arrest at the G1 phase and decreased cell migration, invasion, and wound-healing properties in vitro. A and B, HTH83 cells stably expressing LAMC2 shRNA had increased cells in the G1 phase compared with HTH83 cells stably carrying a scrambled (SCR) shRNA as determined by flow cytometry. C, Western blot showed increased expression of p21/WAF protein in LAMC2 knockdown cells (HTH83 cells). D, Migration assay measured the number of HTH83 cells that migrated through 8-μm pores. LAMC2 shRNA reduced the number of migrated HTH83 cells compared with SCR shRNA. E, LAMC2 shRNA-expressing HTH83 cells displayed a reduced ability to invade through 8-μm pores coated with matrigel as compared with SCR shRNA. F, Wound-healing assay demonstrated that closure of the gap by HTH83 cells stably carrying a LAMC2 shRNA was not completed in 36 hours, whereas HTH83 cells stably carrying a SCR shRNA successfully closed the scratch wound within 36 hours. Cell cycle, migration, and invasion data represent mean ± SD of three independent experiments. *, P ≤ .01; **, P ≤ .001 (Student's t test).