Skip to main content
. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Structure. 2013 Oct 3;21(11):10.1016/j.str.2013.09.001. doi: 10.1016/j.str.2013.09.001

Figure 4. Mutagenesis and Engineering an Artificial T Box-tRNA Interaction System.

Figure 4

(A) EMSAs in which T box mutants are incubated at 0, 0.5, and 1 molar ratio with tRNAGly (g) or tRNAIle (i).

(B) Helical insertions in tRNAGly or Stem I86. Stem I86 constructs for wild-type, 5 bp (Stem I86+5bp), and 11 bp (Stem I86+11bp) insertions are shown in the absence (−) or presence of equimolar tRNAGly constructs for wild-type (g), 5 bp insertion (5), 11 bp insertion (11), or tRNAIle (i) negative control.

(C) Schematics used in (B). Helical insertions highlighted in red.