Fig. 7.

Anesthesia enhances Drp-1 oligomerization in the mitochondria. The samples for Drp-1 oligomer analysis were subjected to a nonreducing gel sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anesthesia (Treat) increases the protein content of the oligomerized form of Drp-1 in the mitochondrial fraction by about 45% compared to sham controls (Cont) (**P = 0.0037; n = 7 pups in the control group; n = 7 pups in the experimental group). The molecular mass standards (in kDa) are shown at the right of the representative Western blots. Drp-1 = dynamin-related protein 1.