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. 2014 Jan 15;141(2):325–334. doi: 10.1242/dev.097097

Fig. 2.

Fig. 2.

Acute effects of GsMTx4 treatment. (A) The AS of an sGMCA embryo before (preinjection) and after control injection. (A′) Microinjection of 10 mM GsMTx4 leads to contraction of AS cells (290 seconds; supplementary material Movie 1). The colored asterisks are for spatial reference. (B) Quantification of AS cell constriction before and after microinjection. (C) No change in GCaMP3 GFP fluorescence in the AS cells (arrow) before and after control injection. (C′) GCaMP3 GFP signal increases in AS cells (arrow) of an embryo microinjected with 10 mM GsMTx4. (D) Ratio of GCaMP fluorescence before and 50 seconds after microinjection. ***P<0.001; **P<0.05; n.s., not significant (*P<0.5) (t-test). For each condition, n=6 embryos. Error bars indicate s.d. Scale bars: 10 μm.