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GsMTx4 blocks closure in a dose-dependent manner. (A) Control injection of sGMCA embryos. (B) 5 mM GsMTx4-injected sGMCA embryos fail to close. Embryos in A and B were injected ∼60 minutes before t=0. Time is shown in minutes. (C) The height of the dorsal opening plotted as a function of time in the control and GsMTx4-injected embryos in A and B. Scale bar: 25 μm.
