Fig. 6.

Knockdown of RPK and dTRPA1 expression disrupts closure. All embryos express sGMCA. (A,A′) Control embryo. (B,C) AS GAL4-driven RNAi against candidate MGCs (B) dtrpA1 and (C) rpk. (B′,C′) Leading edge cells in RNAi-expressing embryos. (D,E) rpk⁵³/Df(3R)ED5092 transheterozygous embryos. (D) Dorsal view of an embryo that failed to complete germband extension (arrowheads). (D′) Leading edge cells of embryo in D. (E) Transheterozygote that completed retraction but failed in closure and head involution. Dashed lines (D,E) delineate AS and LE. Scale bars: 25 μm in E; 10 μm in C′,D′.