Fig. 2. Pretreatment of CSPG blocks morphological changes of WJ-MSCs into neuron-like cells induced by Y27632 and CoCl2 through mediation by PKC. WJ-MSCs were pre-treated with the PKC inhibitors (Go6976 or calphostin C, 100 nM) or GSK3-β inhibitor (SB415286, 10 μM) for 1 h, and then with 10 μg/mL CSPG for 1 h and incubated with 100 μM CoCl2 and 30 μM Y27632 in EGM2 for 72 h. (A) The morphological changes in induced cells were examined under a microscope. Scale bar=100 μm. (B) Total branch length per cell was assessed after 6 or 72 h of treatment with Y27632 and CoCl2. Representative data from three independent experiments performed in triplicate. Y, Y27632; C, CoCl2. Data represent mean ± standard error (n=3). *p<0.05, **p<0.01.
