Table 2.

Mean (±SD) sperm %AB staining, %IAF fluorescence, %DFI, and %HDS in the 3 IUI patient subgroups (normozoospermic, asthenozoospermic, oligo + oligoasthenozoospermic) and controls

Subgroup	N	A	O + OA	Ctl	p-value
n	78	15	9	15
%AB staining	15 ± 9a	23 ± 12b	21 ± 15a,b	12 ± 9a	0.0095
%IAF fluorescence	16 ± 9a	22 ± 12b	21 ± 13a	8 ± 6c	0.0009
%DFI	9 ± 6a	15 ± 7b	11 ± 9a,b	6 ± 3a	0.0017
%HDS	5 ± 2a	6 ± 4a	6 ± 3a	6 ± 3a	>0.05

Values are means ± SD

N normozoospermia, A asthenozoospermia, O + OA oligozoospermia and oligoasthenozoospermia, Ctls fertile controls, AB aniline blue staining (percentage of dark staining), IAF iodoacetimide fluorescence (percentage of intense fluorescence), DFI DNA fragmentation index, HDS high DNA stainability

^a,bDifferent letters indicate significant difference between subgroups (Kruskal-Wallis one-way ANOVA on ranks)