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. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Circulation. 2013 Aug 19;128(12):10.1161/CIRCULATIONAHA.113.003072. doi: 10.1161/CIRCULATIONAHA.113.003072

Figure 6.

Figure 6

Human schistosomiasis-PAH tissue analysis by phospho-Smad2/3 quantification and co-localization with the media vascular compartment, as compared to control tissue from failed lung donors. (A) Representative images showing phospho-Smad2/3 and α-smooth muscle actin (α-SMA) co-localization in the media of normal control and Sc-PAH cases (arrowheads mark double-positive cells; Scale bars: 100 µm). (B) Quantification of phospho-Smad2/3 α-SMA double-positive area in the media (mean ± SE; n = 3–6 cases per group; rank-sum test *P<0.05). (C) Mean fluorescent intensity of phospho-Smad2/3+ pixels in the media (arbitrary units; normalized to average of control = 1; mean ± SE; n = 3–6 cases per group; rank-sum test P=1.0).