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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: ACS Nano. 2013 May 28;7(5):10.1021/nn400702r. doi: 10.1021/nn400702r

Figure 5. Quantification of QD uptake mediated by JB577 peptide variants.

Figure 5

Figure 5

Figure 5

(A) Representative images from the semi-quantitative scoring system for uptake of 550 nm QDs (100 nM QD bearing 75 peptides/QD) and endosomal escape in COS-1 cells. Peptide-QDs were incubated with cells for 2 h, removed and cells cultured for 48 h prior to fixation. (B) Peptide variants with uptake/escape activities comparable to JB577 including: JB865 (Pro9→ Pro12), JB869 (VKIKKP9→amphipathic-[VRLP3]3), JB729 (Palmitoyl→C8F17), SI-09160 (Palmitoyl→ cholesterol), JB872 (Palmitoyl→ farnesyl). JB719 (Chariot peptide) was used as a comparative control peptide. QDs and delivery conditions are as in (a). (C) JB858 directs QD localization to the plasma membrane. PC12-Adh and COS-1 cells were incubated for 1 h with 550 nm QDs (150 nM) assembled with JB858 peptides/QD followed by washing and fixation. (D) Representative confocal micrographs collected at the indicated sectional height through the COS-1 cells from panel (C). Note the imaging and staining at the 7.5 µm height section corresponding to the cells upper membranes. Size bar = 50 µm.