Table 2.
List of repressed genes in intra-colonic environment compared to spent medium with RT-qPCR confirmation
| Locus tag | Gene | Descript | Function | FC microarray (induced in colon) | pvalue microarray | FC RT-qPCR (induced in colon) | pvalue RT- qPCR |
|---|---|---|---|---|---|---|---|
| PFREUD_14940 |
sufS |
Cysteine desulphurases, SufS |
1.2 Transport/binding proteins and lipoproteins |
7,4 |
2,5E-07 |
1,4 |
6,9E-02 |
| PFREUD_19650 |
feoB |
Ferrous iron uptake protein B 9.A.8.1.x |
1.2.3 Transport/binding of inorganic ions |
6,7 |
1,0E-07 |
1,3 |
3,5E-02 |
| PFREUD_20460 |
cycA1 |
D-serine/D-alanine/glycine transporter 2.A.3.1.7 |
1.2.5 Transport/binding of amino-acids |
16,5 |
3,9E-05 |
2,0 |
2,4E-02 |
| PFREUD_05220 |
nuoG |
NADH-quinone oxidoreductase chain G |
1.4 Membrane bioenergetics (electron transport chain and ATP synthase) |
9,9 |
4,3E-08 |
5,3 |
6,0E-03 |
| PFREUD_10590 |
mcoE |
Methylmalonyl-CoA epimerase |
2.1.1 Specific carbohydrate metabolic pathway |
9,3 |
3,0E-07 |
21,7 |
6,8E-02 |
| PFREUD_01040 |
gntK |
Gluconate kinase (Gluconokinase) |
2.1.1 Specific carbohydrate metabolic pathway |
2,9 |
2,9E-05 |
39,5 |
4,3E-02 |
| PFREUD_18870 |
|
Methylmalonyl-CoA carboxytransferase 5S subunit. |
2.1.1 Specific carbohydrate metabolic pathway |
10,3 |
1,1E-07 |
10,0 |
1,3E-02 |
| PFREUD_17320 |
eno1 |
Enolase 1 |
2.1.2 Main glycolytic pathways |
8,4 |
3,6E-07 |
26,3 |
2,0E-03 |
| PFREUD_23890 |
fba2 |
Fructose-bisphosphate aldolase class I |
2.1.2 Main glycolytic pathways |
21,3 |
1,1E-08 |
68,6 |
2,0E-03 |
| PFREUD_05650 |
tuf |
Elongation factor Tu |
3.7.4 Translation elongation |
4,8 |
4,8E-05 |
2,0 |
4,9E-01 |
| PFREUD_17840 |
dnaK1 |
Chaperone protein dnaK 1 |
3.9 Protein folding |
15,5 |
1,5E-07 |
23,3 |
2,5E-03 |
| PFREUD_19250 |
clpB 1 |
Chaperone clpB1 |
3.9 Protein folding |
3,6 |
4,1E-04 |
10,9 |
6,6E-02 |
| PFREUD_22780 | hsp20 1 | Heat shock protein 20 1 (20 kDa chaperone 1) | 3.9 Protein folding | 36,6 | 2,5E-08 | 20,2 | 1,4E-02 |