FIG. 2.

PCR primers used for RT-PCR and real-time RT-PCR. (A) Schematic of the plateau pika VEGF₁₆₅ and VEGF₁₈₉ open reading frames (ORFs). Exons are indicated with 1, 2, 3, 4, 5, 6, 7, and 8. The black, pink, and blue arrows indicate the positions of the PCR primers for cloning, RT-PCR, and real-time RT-PCR analysis, respectively. The red part is exon 6 of the pika VEGF₁₈₉, which is not found in pika VEGF₁₆₅. (B) Primers used in the clone, RT-PCR, and real-time RT-PCR analysis.