Figure 4.

TJ analyses in DSS colitis. A: Western analysis showed increased total expression of claudin-2 protein in the DSS colon of ClC-2^−/− mice. B: Densitometry analysis demonstrates a significant increase in total expression of claudin-2 in DSS colon of ClC-2^−/− mice (^*, different from all other groups, p < 0.05). C: In confocal immunofluorescence, the occludin (green) staining on surface epithelium was diffuse and subapically distributed in the colon of ClC-2^−/− DSS mice compared to the colon of WT DSS mice. The staining of claudin-2 (red) was found to be increased in colonic crypts after DSS administration. The DSS-induced increase in the intensity of claudin-2 staining was higher in ClC-2^−/− DSS mice compared to WT DSS mice. White bar = 50μM.