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Lysis by the mortar–pestle method yields soluble Yck2(SS). Yeast cells, expressing Yck2(wt) or Yck2(SS) from the GAL1 promoter, were lysed by the mortar–pestle method (see Materials and methods), then subsequently treated by Triton X-100 solubilization and centrifugal fractionation, as described for Figure 1A
