Figure 5.

Inclusion of detergent during glass bead disruption reduces the protection from aggregation afforded by membrane association. Glass bead lysates were prepared from yeast cells expressing GAL1-driven Yck2(wt) or Yck2(SS), either by our typical protocol, which adds Triton X-100 subsequent to glass bead disruption, or by an alternative protocol, which included 1% Triton X-100 during the cell disruption step (+ Triton). Subsequent sample processing was as described for Figure 1A, except that no additional detergent was added during the membrane solubilization incubation for the ‘+ Triton’ samples