Table 1. Palmitoyl-proteome change in Hip14-gt versus WT brain.

				Proteomics
Gene Symbol	Description	Change	Ref	ABE	click
Down in HIP14-gt:
Flot1	Flotillin 1; endocytosis; plasma membrane microdomain formation	−36%***	(1)	+	+
Flot2	Flotillin 2; endocytosis; plasma membrane microdomain formation	−36%***	(2)	+	+
Arhgap21	GAP for CDC42	−30%**		+
Ank2	Ankyrin 2; links integral membrane proteins to cytoskeleton; membrane domain role.	−22%**
Syn2	Synapsin 2; associates with cytosolic face of synaptic vesicles	−18%***
Gnal	Gα(olf); Gα olfactory type; stimulates adenylyl cyclase	−17%***	(3)	+
Sept8	Septin 8; GTPase that, together with other septins, forms filamentous, cytoskeletal structures that demarcate polarized plasma membrane structures; neuronal polarity	−13%**		+
Camkv	CAM kinase-like vesicle-associated	−11%**		+
Fasn	Fatty acid synthase (ABE detection may reflect thioester utilization by acyl-carrier function).	−10%***		+	+
Cnp	Cyclic nucleotide phosphodiesterase; highly concentrated in myelin sheaths.	−10%***	(4)	+	+
Gdi2	Guanosine diphosphate dissociation inhibitor; Rab GDI beta.	−10%***			+
Up in Hip14-gt:
Pde2a	Cyclic nucleotide phosphodiesterase, cGMP-activated.	+25%***	(5)	+
Glul	GS; glutamine synthetase	+12%***		+
Lower significance:
Add1	Adducin 1; α-adducin; heterodimer with γ-adducin; membrane-cytoskeleton associated; promotes assembly of actin/spectrin network; binds calmodulin.	−19%*
Slc44a1	CTL1; choline transporter-like 1; multi-pass membrane protein; component of mitochondrial outer membrane; may be involved in myelin synthesis.	−15%*		+
Rap2a	Small RAS family G protein; regulates dendrite extension and arborization; farnesylated and palmitoylated.	−14%*	(6)	+	+
Mtap1b	Microtubule-associated protein; role in neurite outgrowth.	−13%*			+
Gpd2	Glycerol-3—phosphate dehydrogenase 2; mitochondrial inner membrane localization.	−11%*
Pfkl	Phosphofructokinase, liver.	−10%*

The proteins showing significant change (* P < 0.05; ** P < 0.01; *** P < 0.001) of greater than 10% in the Hip14-gt brain palmitoyl-proteome relative to the WT brain palmitoyl-proteome are listed. The three rightmost columns provide the published evidence supporting palmitoylation documentation for each protein, including either a specific reference (see below), or a “+” indication that the protein was identified as likely palmitoylated in prior ABE- or click-based proteomic analyses (see Table S1 for the specific references to these proteomic studies and for the expanded ABE/SILAM data set). See Figure S2 for graphical depictions of the peptide data for the proteins reported within this table.

References:

⁽¹⁾(Morrow et al., 2002);

⁽²⁾(Neumann-Giesen et al., 2004);

⁽³⁾(Linder et al., 1993);

⁽⁴⁾(Agrawal et al., 1990);

⁽⁵⁾(Russwurm et al., 2009);

⁽⁶⁾(Beranger et al., 1991).