. Author manuscript; available in PMC: 2014 Nov 21.

Published in final edited form as: Chem Biol. 2013 Nov 7;20(11):10.1016/j.chembiol.2013.09.018. doi: 10.1016/j.chembiol.2013.09.018

Table 3.

Palmitoyl-proteome change in YAC128 relative to WT littermate brain.

				proteomics
Gene Symbol	Description	Change	Ref	ABE	click
Down in YAC128:
Car2	CA II; carbonic anhydrase II; mediates the interconversion of CO₂ and H₂O to H⁺ and HCO₃⁻; localized in brain mainly to oligodendrocytes and astrocytes.	−42%***			+
Glul	GS; glutamine synthetase; converts glutamate to glutamine; role implicated in excitotoxicity; localized in brain mainly to astrocytes.	−29%***		+
Pde10a	cAMP and cGMP 3′,5′-cyclic phosphodiesterase 10A, cAMP-inhibited; localized mainly to striatum.	−27%***	⁽¹⁾	+
Vdac3	Voltage-dependent anion-selective channel protein 3; mitochondrial outer membrane porin.	−17%***		+	+
Uqcrc1	Cytochrome b-c1 complex protein 1, mitochondrial	−16%**			+
Cnp	CNP II; 2′,3′-cyclic-nucleotide 3′-phosphodiesterase2; localizes to oligodendrocyte myelin membranes	−14%***	⁽²⁾	+	+
Atp2b1	PMCA1; plasma membrane calcium-transporting ATPase 1.	−14%***		+
Atp2b4	PMCA4; plasma membrane calcium-transporting ATPase 4.	−11%**		+	+
Slc1a2	GLT-1; EAAT2; glial high affinity glutamate transporter; astrocyte protein; mediates retrieval of synaptic glutamate; implicated role in excitotoxicity.	−11%**	⁽³⁾	+
Up in YAC128:
Gnas	Gαs; Gα heterotrimeric G protein component; stimulates adenylyl cyclase.	+10%***	⁽⁴⁾	+	+
Lower Significance:
Igsf8	EWI-2; PGRL; CD316; immunoglobulin superfamily member; interacts with tetraspannins to regulate cell migration.	−27%*	⁽⁵⁾	+	+
Hadhb	Trifunctional enzyme (hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase), subunit beta, mitochondrial.	−24%*		+	+
Slc1a3	GLAST; EAAT1; glial high affinity glutamate transporter; astrocyte protein; mediates retrieval of synaptic glutamate; implicated role in excitotoxicity.	−14%*		+
Dlg4	PSD-95; SAP-90; MAGK scaffolding protein that clusters NMDA glutamate receptors at synapse.	−13%*	⁽⁶⁾	+	+
Mog	Myelin oligodendrocyte glycoprotein; IgG superfamily; type 1 TM protein localizing to outer layer of myelin sheath; auto-antigen in multiple sclerosis; causal mutation in familial form of narcolepsy.	−13%*		+
Gria3	GluR3; GluA3; AMPA glutamate receptor subunit.	−10%*	⁽⁷⁾	+
Arvcf	Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog.	+33%*		+
Uba1	Ubiquitin-like modifier-activating enzyme 1; ubiquitin E1 enzyme; likely detected by ABE due to its transient thioester linkage to ubiquitin.	+17%*		+	+
Arhgap21	GAP for CDC42.	+11%*		+

Data is reported for the proteins that show significant change (* P < 0.05; ** P < 0.01; *** P < 0.001) of greater than 10% in the YAC128 palmitoyl-proteome relative to the WT palmitoyl-proteome. The three rightmost columns provide the published evidence supporting palmitoylation documentation for each protein, including either a specific reference (see below), or a “+” indication that the protein was identified as likely palmitoylated in prior ABE- or click-based proteomic analyses (see Table S2 for references to the relevant proteomic studies and for the expanded ABE/SILAM dataset). Proteins that are expressed predominantly in either oligodendrocytes or astrocytes are underlined.

References:

⁽¹⁾

(Charych et al., 2010);

⁽²⁾

(Agrawal et al., 1990);

⁽³⁾

(Huang et al., 2010);

⁽⁴⁾

(Linder et al., 1993);

⁽⁵⁾

(He et al., 2011);

⁽⁶⁾

(Topinka and Bredt, 1998);

⁽⁷⁾

(Hayashi et al., 2005).