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Primer sequences used for NRAS and BRAF sequencing analysis
| Gene | Primer Sequence |
|---|---|
| NRAS exon 2 | Forward: 5’-GAACCAAATGGAAGGTCACA-3’ Reverse: 5’-TGGGTAAAGATGATCCGACA-3’ |
| NRAS exon 3 | Forward: 5’-GGTGAAACCTGTTTGTTGGA -3’ Reverse: 5’-AACCTAAAACCAACTCTTCCCA-3’ |
| BRAF exon 11 | Forward: 5’-TCCCTCTCAGGCATAAGGTAA-3’ Reverse: 5’-CGAACAGTGAATATTTCCTTTGAT-3’ |
| BRAF exon 15 | Forward: 5’-TCATAATGCTTGCTCTGATAGGA-3’ Reverse: 5’-GGCCAAAAATTTAATCAGTGGA-3’ |
