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. 2013 Nov 14;13:100. doi: 10.1186/1472-6750-13-100

Figure 6.

Figure 6

SDS-PAGE and zymogram gels of CmINV. (a) Silver-stained 10% polyacrylamide gel of crude extract of S. cerevisiae BY4742[CmINV] in lane 2. Lane 3 is a reference S. cerevisiae BY4742[pJC1] strain containing the empty vector pJC1. (b) Silver-stained 10% polyacrylamide gel of purified CmINV. Lane 2 contains the de-N-glycosylated version (lower band corresponds to PNGase F). Lane 3 contains the un-de-N-glycosylated version of purified CmINV. (c) Zymogram gel of purified CmINV. Lanes were loaded similar to gel b. The protein molecular weight markers PageRuler unstained Protein Ladder (lanes 1 in a and b) and PageRuler Prestained Protein Ladder (Thermo Scientific) (lane 1 in c) were used in SDS-PAGE and Zymogram analyses, respectively.