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. 2014 Jan 3;9(1):e81126. doi: 10.1371/journal.pone.0081126

Figure 7. mGluR1 regulates TNBC migration and invasion.

Figure 7

MCF10AT1 or MCF10CA1d cells were transduced either with pLenti-GRM1, pLenti-LacZ, shGRM1, or NS control. 2.5×105 cells were plated on polycarbonate containing cell inserts and migration or invasion towards chemoattractant (10% serum) was measured after 24 hours. For invasion assays, polycarbonate membranes were coated with reconstituted basement membrane. A. mGluR1 stimulates MCF10AT1 migration. GRM1 overexpression in MCF10AT1 cells significantly increased migration of the cells compared to the control LacZ cells, which was reversed (B) by silencing with shGRM1. C. MCF10AT1 invasion. GRM1 overexpression significantly stimulated the invasion of MCF10AT1 cells through polycarbonate membranes coated with reconstituted basement membrane. D. MCF10CA1d invasion. shGRM1 knockdown inhibited the invasion of malignant MCF10CA1d cells compared to the NS transduced cells. Results are recorded as the mean ± SEM of three experiments, where *p<0.05 compared to control. Legend: NS = nonsilencing control; shGRM1 = shRNA against GRM1; LacZ = pLenti-LacZ; GRM1 = pLenti-GRM1.