. 2014 Jan 3;9(1):e83955. doi: 10.1371/journal.pone.0083955

Table 1. Proteomic profiling of Evs secreted by breast primary human mammospheres.

OH^a	E2^a	TX^a	PROTEIN ID	PROTEIN NAME	IPA^b
					Can	Prol	Dev
				Membrane-associated proteins
36	64	59	TRFL_HUMAN	Lactotransferrin	x	x	x
	9		MFGM_HUMAN	Lactadherin/Milk fat globule-EGF factor 8	x	x	x
	13	8	ANXA2_HUMAN	Annexin A2	x	x	x
	23		PROM1_HUMAN	Prominin-1/CD133	x
	17		AMPN_HUMAN	Aminopeptidase N/CD13	x	x	x
	2		ERGI2_HUMAN	ER Golgi intermediate compartment protein 2
	6		RAB15_HUMAN	Ras related protein Rab 15			x
	8		RUFY2_HUMAN	RUN and FYVE domain containing protein 2	x
	11		HS90A_HUMAN	Heat shock protein HSP 90-alpha	x	x	x
		3	BAK_HUMAN	Bcl 2 homologous antagonist killer	x	x	x
				Enzymes
3	12	8	G3P_HUMAN	Glyceraldehyde-3-phosphate dehydrogenase	x	x	x
8	11		ALDOB_HUMAN	Fructose-bisphosphate aldolase B	x	x
	8		ALDOC_HUMAN	Fructose bisphosphate aldolase C	x	x
2	3		TPIS_HUMAN	Triosephosphate isomerase	x
	6		KPYM_HUMAN	Pyruvate kinase isozymes M1/M2	x	x
	10	13	DHE3_HUMAN	Glutamate dehydrogenase 1, mitochondrial	x	x	x
	4		HCD2_HUMAN	3-hydroxyacyl-CoA dehydrogenase type-2	x	x
2	12	8	SAHH_HUMAN	Adenosylhomocysteinase		x	x
	11	4	AL1A1_HUMAN	Retinal dehydrogenase 1	x	x	x
	8		FTHFD_HUMAN	10 formyltetrahydrofolate dehydrogenase	x
		7	DPYS_HUMAN	Dihydropyrimidinase
	4		ACADS_HUMAN	Short-chain acyl-CoA dehydrogenase
	6		THIM_HUMAN	3-ketoacyl-CoA thiolase, mitochondrial
		3	NFS1_HUMAN	Cysteine desulfurase mitochondrial		x
	7		CATA_HUMAN	Catalase	x	x	x
				Miscellaneous
11	20	17	ALBU_HUMAN	Serum albumin	x	x	x
3	9	6	UBIQ_HUMAN	Ubiquitin	x
	5		PSD10_HUMAN	26S proteasome non-ATPase reg sub10		x
		13	ACTA_HUMAN	Actin, aortic smooth muscle	x
12	28		ACTB_HUMAN	Actin, cytoplasmic 1	x	x	x
	26		ACTG_HUMAN	Actin cytoplasmic 2	x	x	x
	16		CO6A1_HUMAN	Collagen alpha-1(VI) chain		x	x
	10		CO6A2_HUMAN	Collagen alpha-2(VI) chain		x	x
		4	TRY6_HUMAN	Putative trypsin 6
3			H2A1B_HUMAN	Histone H2A type 1 B
	20	6	H2AX_HUMAN	Histone H2A x		x	x
6	10	15	H2B1K_HUMAN	Histone H2B type 1 K	x
21			H31_HUMAN	Histone H3.1
	17	36	H33_HUMAN	Histone H3.3
12	15	10	H4_HUMAN	Histone H4
11	9	8	H13_HUMAN	Histone H1.3		x
6	4	4	H14_HUMAN	Histone H1.4		x

^a Number of peptides identified in the mass spectrometry analysis.

^b Functional categorization using Ingenuity Pathway Analysis software. The functional categories more represented in the proteomics analysis were Cancer (Can), Proliferation (Prol) (including cellular growth, cell cycle and cell death) and Development (Dev) (including cell signaling, cellular assembly and organization, tissue remodeling and maintenance).