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. 2014 Jan 3;9(1):e84392. doi: 10.1371/journal.pone.0084392

Figure 6. Quantitative analysis of colocalization between HRES-1/Rab4 and mitochondria in HeLa cells transfected with eGFP-tagged HRES-1/Rab4 isoforms and FP650-LC3.

Figure 6

Autophagy was induced by starvation (Star) or treatment with rapamycin (Rapa) in the presence or absence of bafilomycin A1 (Baf). A, Colocalization of HRES-1/Rab4 and MTDR-stained mitochondria relative to the total mitochondrial pool. B, Colocalization of HRES-1/Rab4 and MTDR-stained mitochondria relative to the total HRES-1/Rab4 pool. Bars represent mean ± SEM. Data represent mean ± SEM of 6–29 cells acquired in 3 independent experiments. * indicates p values<0.05 reflecting comparison to control cells among cell cultures transfected with the same construct using paired two-tailed t-tests; brackets connecting bars within each construct also reflect comparison with paired two-tailed t-tests. Brackets connecting bars between constructs reflect p<0.05 using ANOVA followed by Bonferroni's post-test.