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. 2014 Jan 3;9(1):e84550. doi: 10.1371/journal.pone.0084550

Figure 4. PTEN deletion in L6 cells reversed the effect of silymarin on insulin sensitivity and glucose uptake.

Figure 4

Cells were transfected for 48(A). Cells treated with 1 µM silymarin for 6 h with or without specific PTEN siRNA were exposed or not to 0.1 µM insulin. Equal amounts of the total lysates of each group were immunoblotted with anti-phospho-Akt (pAkt) or Akt (B). Cells were transfected for 48 h with 50 pmol PTEN siRNA or scramble siRNA were treated with 1 µM silymarin 6 h with or without specific PTEN siRNA and exposed or not to 0.1 µM insulin for 30 min. The L6 cells were then treated with 200 µM 2-NBDG for 1 h to evaluate the glucose uptake ability of each group (C). Values represent means ± standard error of mean for 3 independent.