Figure 2. JakI-1 or dasatinib reduces proliferation and viability of PVTL-1 cells.

A, B) PVTL-1 cells were plated at 5×10⁵ cells/ml in RPMI1640 medium with 10% FCS and 1 µM JakI-1, 100 nM dasatinib, or 5 µM imatinib, as indicated, for indicated days. Viable cell numbers and viability were counted and plotted in A and B, respectively. C, D) PVTL-1 or HEL cells, as indicated, were cultured with indicated concentrations of JakI-1 (C) or dasatinib (D) for 3 days and viable cell numbers were measured by XTT colorimetric assay. Each data point represents the mean of triplicate determinations, with error bars indicating standard errors, and is expressed as a percentage of cell numbers without inhibitors. E) PVTL-1 cells were cultured with indicated concentrations of JakI-1, dasatinib, or both, as indicated, for 3 days and analyzed by XTT colorimetric assay. Combination index (CI) values obtained by the method of Chou and Talalay [21] are indicated.