Figure 3. JakI-1 or dasatinib induces apoptosis involving loss of mitochondrial membrane potential and activation of caspase-3 in PVTL-1 cells.

PVTL-1 cells were untreated as control (Cont.) or treated with 1 µM JakI-1 or 10 nM dasatinib, as indicated, for 48 h. Cells were then analyzed by flow cytometry for mitochondrial membrane potential with DiOC6, activation of caspase-3 with the cleavage-specific antibody, surface expression of Annexin V with anti-Annexin V, and cellular DNA content with PI, as indicated. Percentages of cells affected are indicated.