Table 1. Predictions of the number of isolates of each pospiviroid species that will be detected using universal and species-specific assays of the Luminex MagPlex-TAG pospiviroid array.

Viroid species	Acronym	Number ofGenBanksequencesb	Number of isolates predicted to be detected with each assayc,d
			CSVd	CEVd	CLVd	IrVd-1	PCFVd	PSTVd	TASVd	TCDVd	TPMVd	PospUni
Chrysanthemum stunt viroid	CSVd	50	50									48
Citrus exocortis viroid	CEVd	204		204								200
Columnea latent viroid	CLVd	74			71							N/A
Iresine viroid 1	IrVd-1	7				7						7
Pepper chat fruit viroid	PCFVd	38					38					38
Potato spindle tuber viroid	PSTVd	225						218				222
Tomato apical stunt viroid	TASVd	15							14			15
Tomato chlorotic dwarf viroid	TCDVd	13								13		13
Tomato planta macho viroid a	TPMVd	16									15	16

^a For Tomato planta macho viroid primer design, sequences of Mexican papita viroid were included to give a total of 16 sequences for this conspecific species.

^b For analysis, only full-length sequences available on GenBank for each species at the time of array design (May 2011) were included.

^c Acronyms refer to assays of the multiplexed array, specific for: CSVd - Chrysanthemum stunt viroid; CEVd - Citrus exocortis viroid; CLVd - Columnea latent viroid; IrVd-1 - Iresine viroid 1; PCFVd - Pepper chat fruit viroid; PSTVd - Potato spindle tuber viroid; TASVd - Tomato apical stunt viroid; TCDVd - Tomato chlorotic dwarf viroid; TPMVd - Tomato planta macho viroid; PospUni - pospiviroid universal assay.

^d Predictions based on the maximum number of mismatches of TSPE primers n = 1. No mismatches were allowed in the final three positions of the 3′ end of TSPE primer for a given assay.