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. 2014 Jan 3;9(1):e85397. doi: 10.1371/journal.pone.0085397

Figure 7. Colon cancer stem cell marker expression in high density cultures as shown by western blotting evaluation.

Figure 7

High density cultures of HCT116 and HCT116+ch3 (left lanel, A, B, C) and of HCT116R and HCT116+ch3R (right panel, A, B, C) cells were either left untreated or were treated with 5-FU (5 µM), curcumin (20 µM), or 5-FU/curcumin in combination (0.1/5 µM). Cultures were evaluated after 3 days and whole cell lysates prepared and analyzed by western blotting and quantitative densitometry for expression of CD133, CD44 and ALDH1. Western blots shown are representative of three independent experiments. The housekeeping protein β-actin served as a positive loading control in all experiments. Significant values are marked with (*).