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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Innate Immun. 2013 Jul 24;20(4):377–389. doi: 10.1177/1753425913493453

Figure 3. NOD activation by N. gonorrhoeae alters chemokine induction in cervical epithelial cells.

Figure 3

HeLa cells were primed overnight with IFN-γ (100 U/ml), except as indicated in (A), followed by incubation with live Neisseria gonorrhoeae (GC) or heat killed Neisseria gonorrhoeae (HK GC) (both MOI 50:1), the NOD1 ligand C12iEDAP (10 μg/ml), human TNF-α (100 ng/ml), LPS (100 ng/ml), P3CSK4 (100 ng/ml), or medium only (M). Where indicated, the RIPK2 inhibitor SB 203580 (B) or the Nod1 inhibitor ML130 (Fig. 3C) was added 1 hour prior to treatment at the indicated concentrations. Cell supernatants were collected after 22 hrs and analyzed for the presence of IL-8 by ELISA. *=p<0.05; **=p<0.005; ***=p<0.0005; NS=not statistically significant; ND=not detected. All conditions were tested in triplicates. Each experiment was repeated 2 times with similar findings.