Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 2;14:1. doi: 10.1186/1471-2407-14-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Xiong et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Reducing P4HA2 expression or inhibiting its activity impairs collagen deposition. (A) Western blot analysis of collagen I and IV in conditional media generated from the same amount of control and P4HA2-silenced (sh-P4HA2-1) T4-2 cells; bar graph shows quantification of Western blot results (*p < 0.05; **p < 0.01) (B) Immunofluorescence staining of collagen I (green) and IV (green) in control and P4HA2-silenced T4-2 cells. P4HA2 knockdown (sh-P4HA2-1) T4-2 cells showed impaired collagen I and IV protein deposition. (C) Immunofluorescence staining of collagen I (green) and IV (green) in control and 1,4-DPAC treated T4-2 cells in 3D culture. T4-2 cells treated with 1,4-DPAC showed lower expression of collagen I and IV (scale bar, 50 μm).