Table 2.
Ratios of COX-2 mRNA expression in paired colorectal tumor/normal mucosa, normalized with respect to tissue weight or to the levels of the housekeeping genes B2M or GAPDH
| Tissue weight | B2M | GAPDH | |
|---|---|---|---|
| Number of pairs |
N = 60 |
N = 60 |
N = 55* |
| Mean ratio |
21.6 |
16.1 |
7.5 |
| Range |
0.01-595.3 |
0.01-525.5 |
0.00-150.9 |
| Ratio >1 (%)** |
46 (77%) |
42 (70%) |
22 (40%) |
| Ratio <1 (%)*** | 14 (23%) | 18 (30%) | 33 (60%) |
B2M, beta-2 microglobulin.
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
*Note that GAPDH was not detectable or the analysis did not succeed in 5 cases.
**Ratio > 1 means that COX-2 expression is higher in colorectal tumor tissue compared to paired normal mucosa.
***Ratio < 1 means that COX-2 expression is lower in colorectal tumor tissue compared to paired normal mucosa.