Table 1.
Expression levels of selected biomass-conversion enzyme-genes in the three sections of the fungus garden
| Tag id | Enzyme function deduced from BLAST hits | CAZy family a |
Top |
Middle |
Bottom |
Test for equal level P | Fold up, bottom relative to top (95% CI) |
|---|---|---|---|---|---|---|---|
| Mean tag count (95% CI) | |||||||
| 23641 |
Endocellulase |
GH5, CBM1 |
6 (0, 14) |
41 (0, 123) |
370 (150, 591) |
0.021 |
57.9 (25.21, 268.15) |
| 16339 |
Endocellulase |
GH5 |
96 (13, 178) |
28 (0, 60) |
140 (56, 224) |
0.025 |
1.5 (0.71, 3.98) |
| 10483 |
Cellobiohydrolase (CBHI-I) |
GH7, CBM1 |
14 (0, 28) |
56 (0, 158) |
524 (349, 698) |
2.2e-3 |
36.4 (20.10, 107.96) |
| 24891 |
Cellobiohydrolase (CBHI-II) |
GH7 |
449 (13, 885) |
51 (0, 137) |
1211 (911, 1512) |
2.2e-4 |
2.7 (1.53, 8.43) |
| 20092 |
β-Glucosidase, intracellular |
GH3 |
23 (1, 45) |
12 (0, 33) |
58 (38, 79) |
7.2e-3 |
2.6 (1.41, 7.54) |
| 2990 |
Lytic polysaccharide monooxygenase |
AA9 |
0 |
21 (0, 60) |
96 (53, 140) |
0 |
NA |
| 323 |
Xylanase |
GH10, CBM1 |
7 (0, 21) |
92 (0, 274) |
669 (360, 977) |
8.0e-3 |
101.3 (35.68, 1089.35) |
| 7614 |
Acetyl xylan esterase |
CE1, CBM1 |
88 (38, 137) |
175 (0, 395) |
1168 (917, 1419) |
3.0e-4 |
13.3 (9.12, 22.92) |
| 18781 |
Xyloglucanase |
GH12 |
16 (1, 31) |
95 (70, 119) |
141 (0, 287) |
4.3e-4 |
8.8 (2.32, 29.17) |
| 29382 |
Xyloglucanase |
GH74, CBM1 |
85 (5, 164) |
41 (0, 100) |
540 (324, 756) |
2.0e-3 |
6.4 (3.48, 18.33) |
| 17822 |
Pectinesterase |
CE8 |
77 (0, 159) |
39 (0, 81) |
209 (128, 290) |
4.9e-3 |
2.7 (1.43, 10.17) |
| 24998 |
Pectinesteraseb |
CE8 |
529 (295, 763) |
435 (297, 572) |
698 (304, 1092) |
0.25 |
1.3 (0.75, 2.19) |
| 13889 |
Polygalacturonaseb |
GH28 |
403 (273, 533) |
386 (244, 527) |
686 (418, 954) |
0.075 |
1.7 (1.16, 2.43) |
| 16820 |
Pectate lyaseb |
PL1 |
34 (11, 56) |
19 (0, 57) |
189 (10, 368) |
0.11 |
5.6 (1.88, 12.58) |
| 25523 |
Pectate lyase |
PL1 |
222 (74, 370) |
146 (0, 381) |
704 (547, 860) |
8.5e-4 |
3.2 (2.06, 6.01) |
| 12708 |
Pectate lyase |
PL3 |
6 (0, 17) |
54 (0, 160) |
655 (398, 912) |
4.1e-3 |
116.9 (45.09, 1220.07) |
| 29370 |
Rhamnogalacturonan lyase |
PL4 |
208 (87, 329) |
259 (125, 392) |
757 (477, 1036) |
6.0e-3 |
3.6 (2.28, 6.45) |
| 17541 |
Endo-1,5-α-L-arabinanase |
GH43 |
141 (22, 260) |
81 (31, 131) |
204 (110, 297) |
0.043 |
1.4 (0.79, 3.63) |
| 17220 |
Glucoamylase |
GH15, CBM20 |
32 (1, 62) |
35 (0, 107) |
130 (113, 146) |
8.6e-4 |
4.1 (2.40, 11.80) |
| 26445 |
α-Galactosidase |
GH27 |
162 (79, 245) |
163 (93, 233) |
660 (578, 742) |
5.7e-6 |
4.1 (2.96, 6.40) |
| 10080 |
Cutinase |
- |
433 (0, 889) |
367 (156, 577) |
960 (743, 1177) |
2.4e-3 |
2.2 (1.24, 7.86) |
| 28797 |
Cutinase |
- |
211 (0, 444) |
242 (0, 555) |
468 (340, 596) |
0.067 |
2.0 (1.18, 9.25) |
| 14730 |
Esterase/lipase |
- |
2 (0, 7) |
31 (0, 88) |
357 (298, 416) |
9.9e-5 |
148.6 (65.90, 1290.24) |
| 1743 | Lipase | - | 144 (28, 259) | 595 (424, 765) | 718 (423, 1013) | 6.7e-4 | 5.0 (2.78, 12.01) |
aCarbohydrate-Active Enzymes family [27]: GH, glycoside hydrolase; AA, auxiliary activity; CBM, carbohydrate-binding module; CE, carbohydrate esterase; PL, polysaccharide lyase.
bIdentified by Schiøtt et al. [7] [GenBank:HQ174765-HQ174767].