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. 2013 Nov 1;3(11):e156. doi: 10.1038/bcj.2013.53

Figure 1.

Figure 1

cMYC/KRAS12V can transform BaF3 cells independent of IL3 and transduce T2 B cells in vitro. (a) Workflow for generation of the adoptive plasmacytoma mouse model. (b) Schematic diagram of MSCV-based retroviral vectors: MIG, cMYC, KRAS12V and cMYC/KRAS12V. (c) Western blot analysis showed expression of MIG, cMYC, KRAS12V and cMYC/KRAS12V in transfected 293T cells. Total protein was analyzed against human MYC (upper) and RAS (middle); GAPDH (lower) served as a loading control. (d) KRAS12V and cMYC/KRAS12V drove BaF3 cells to grow independent of IL3 in vitro. Representative cells from two independent experiments are shown. (e) The purification of mouse IgM+ spleen B cells after micro-bead isolation is shown (left panel). The cell population transited to T2 B-cell subset (IgM+B220+ CD38+IgD+) after stimulation with LPS and mIL4 for 48 h (right three panels). Assays were independently and repeatedly performed. (f) Colonies in methylcellulose assays were observed only in cells transduced by cMYC/KRAS12V but not other groups. Three independent experiments were performed.