Skip to main content
. 2013 Nov 1;3(11):e156. doi: 10.1038/bcj.2013.53

Figure 4.

Figure 4

Evidence of clonality and hypermutation of expressed Ig genes in cMYC/KRAS12V-induced plasmacytoma mice. (a) Serum protein electrophoresis (SPEP) in serum and ascites from cMYC/KRAS12V-induced plasmacytoma mice. The bands are indicated with arrows. Samples were obtained from all the group animals as labeled (n=5 each group). (b) Ig isoform identification. Ascites were collected from plasmacytoma mice and analyzed with Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit. The Ig isoforms (n=10) are represented by color labeled bands as indicated. (c) Southern blot analysis for clonotypic IgH -chain rearrangement in plasmacytoma cells. Genomic DNA was isolated from IgM+ B cells (sample 1) or cMYC/KRAS12V-induced plasmacytoma cells (samples 2–7), digested with EcoRI restriction enzyme and hybridized with murine JH4 probe. GL denotes germline band.