Skip to main content
. 2013 Nov 29;3(11):e166. doi: 10.1038/bcj.2013.63

Figure 5.

Figure 5

OPB-31121 overcame autocrine-induced FLT3 inhibitor resistance. (a) MOLM13 cells were treated with 100 nM sunitinib, 100 nM OPB-31121 and 100 ng/ml FLT3 ligand as indicated for 4 h. Then, cells were lysed and subjected to IB with the indicated antibodies. (b) FLT3 ligand reduced sunitinib sensitivity in MOLM13 cells. MOLM13 cells were cultured with the indicated dose of sunitinib with or without 100 ng/ml FLT3 ligand. Cell proliferation was analyzed by MTT assay after 48 h culture. MTT values were plotted as relative values to the value of the cells without FLT3 ligand and sunitinib. The average value of three experiments was plotted with standard deviation. (c) FLT3 ligand did not affect the sensitivity to OPB-31121 in MOLM13 cells. Sensitivity of OPB-31121 was measured as in (b) using OPB-31121 instead of sunitinib.