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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biotechniques. 2013 Nov;55(5):10.2144/000114100. doi: 10.2144/000114100

Figure 1. Structures of pFA6a derivatives described in this study.

Figure 1

(A) pFA6a-12Pk-MX6 modules used to amplify gene cassettes for C-terminal 12Pk tagging. The pFA6a backbone is only shown for pFA6a-12Pk-kanMX6. Arrows inside the boxes indicate the transcriptional direction of selection marker genes. Primers for the one-step PCR method are shown as arrows outside the boxes (not to scale). (B) pFA6a-G11/9–5FLAG-kanMX6 modules for C-terminal G11/9–5FLAG tagging. (C) pFA6a derivative for C-terminal GFP and mRFP tagging. (D) Vectors for gene deletion using nutritional markers. All of these modules are based on the pFA6a system described by Bähler et al. (2). Plasmid names and PCR product sizes for both one-step and two-step PCR methods are shown. amp, ampicillin resistant gene; ori, replication origin; TADH, ADH1 gene terminator; PTEF, TEF gene promoter.