Figure 3.

Expression changes in mock-treated and infected DBA/2J (left column) and C57BL/6J (right column) mice in the 10 target host mRNAs in the 5-day time course of IAV infection. Analysis of the same data set as used for Figure 2. Panels show fold change expression, determined by qRT-PCR, of Fos(A, B), Retnla(C, D), Irg1(E, F), Il6(G, H), Il1b(I, J), Cxcl10(K, L), Stat1(M, N), Ifng(O, P), Ifnl2(Q, R), and Mx1(S, T) mRNA. Fold change data of Ifnl2 represent an underestimation, as a Ct of 40 was assigned to all samples with Ct >40 (see Methods section). Solid lines, mice intranasally infected with 1x10³ ffu of IAV strain PR8_Mun; interrupted lines, mice undergoing the same anesthesia/infection procedure except that buffer only, not containing virus, was used for intranasal installation (mock treatment). *, p ≤0.05 for difference between infected mice at the given time point with respect to t = 0 h; ∆, p ≤0.05 for difference between mock treated and control (t = 0 h) mice; ‡, p ≤0.05 for difference between infected and mock treated mice at the given time point. All p values were determined with Tukey’s test.